bioGenousTM Human Airway (Differentiation) Organoid Kit is a serum-free culture medium designed for the differentiation of human airway organoids (hAOs) derived from human airway stem cells. When cultured in differentiation conditions for 10-14 days, airway organoids develop well-defined luminal structures, within which extensive ciliary beating or accumulation of secretory material can be observed under the microscope. Differentiated hAOs exhibit key functional and structural features of the mature airway epithelium, including high expression of tubulin in motile cilia and MUC5AC in mucus-secreting goblet cells. In combination withbioGenousTM Human Airway (Expansion) Organoid Kit, this differentiation system enables robust and reproducible generation of airway epithelium in vitro, supporting a wide range of applications in respiratory research, toxicology, drug discovery, and regenerative medicine.