9-氨基-6-氯-2-甲氧基吖啶

9-Amino-6-chloro-2-methoxyacridine (ACMA)  

產品簡介：

9-氨基-6-氯-2-甲氧基吖啶（9-Amino-6-chloro-2-methoxyacridine, ACMA）是一種細胞通透性的熒光探針，一種DNA嵌入劑，選擇性結合poly (dA-dT)序列，隨著插入鳥苷熒光壽命逐漸減少。ACMA能用于DNA標記，激發/發射波長為411/475n，可采用大多數紫外光源激發，因而兼容更短和更長波長的染料。ACMA熒光依賴pH，并在形成pH梯度時淬滅，這一特征被用于基于動物和植物的研究中。ACMA也抑制膽堿酯酶活性，Ki為49nM。

產品特性：

1）  CAS NO：3548-09-2

2）  化學名：6-chloro-2-methoxy-9-acridinamine

3）  同義名：ACMA，NSC 15300

4）  分子式：C₁₄H₁₁ClN₂O

5）  分子量：258.7

6）  外觀：固體

7）  純度：≥98%

8）  Ex/Em：411/475nm

9）  溶解性：溶于甲醇

保存條件: 

-20°C避光干燥保存，2年有效。

產品使用： 染色示例（來自文獻，僅作參考）

Ref 1）Jin, R., He, S., Black, K.A. et al. Ion currents through Kir potassium channels are gated by anionic lipids. Nat Commun 13, 490(2022).

https://doi.org/10.1038/s41467-022-28148-4

Assay：Liposomal fluorimetric assay 

Method：The pH sensitive dye 9-amino-chloro-2-methoxyacridine (ACMA) was added to a final concentration of 2?μM from a freshly prepared 200?μM stock in 100% (v/v) ethanol. Fluorescence emission at 483?nm (excitation at 419?nm) was monitored over time with pathlengths of 2 and 10?mm for excitation and emission, respectively.

Ref 2）Furrer EM, Ronchetti MF, Verrey F, Pos KM. Functional characterization of a NapA Na(+)/H(+) antiporter from Thermus thermophilus. FEBS Lett. 2007 Feb 6;581(3):572-8. doi: 10.1016/j.febslet.2006.12.059. Epub 2007 Jan 17. PMID: 17254570.

Assay：9-Amino-6-chloro-2-methoxyacridine (ACMA) fluorescence assay to detect Na+/H+ antiport activity

Method：Na+/H+ antiporter activity was measured in a thermostated cuvette containing a continuously stirred suspension of 2 ml of 10 mM Tris–HEPES at various pH (6.0–8.0), 140 mM choline chloride, 5 mM MgCl2, 2 μM ACMA and membranes (200 μg of protein). The changes in pH inside the membranes were monitored by following the (de)quenching of ACMA fluorescence (excitation 410 nm; emission 480 nm).

注意事項：

1.      熒光染料均存在淬滅問題，請盡量注意避光，以減緩熒光淬滅。

2.      為了您的安全和健康，請穿實驗服并戴一次性手套操作。