Mirus   TransIT-LT1 Transfection Reagent

Description描述

l  Broad Spectrum DNA Delivery – Utilize one transfection reagent and protocol for a variety of cells
廣譜 DNA 傳遞——使用一種轉(zhuǎn)染試劑和方案適用于多種細(xì)胞

l  Low Cellular Toxicity – Maintain cell density and reduce experimental biases低細(xì)胞毒性——保持細(xì)胞密度并減少實(shí)驗(yàn)偏差

l  High Efficiency Delivery – Achieve expression in a large population of cells for experimental success
高效傳遞——在大量細(xì)胞中實(shí)現(xiàn)表達(dá)，實(shí)驗(yàn)成功

l  Deliver Single or Multiple Plasmids – Suitable for applications such as virus production
交付單個(gè)或多個(gè)質(zhì)粒——適用于病毒生產(chǎn)等應(yīng)用

保存條件: 

Store at 4°C

? 通過編碼shRNA的質(zhì)粒DNA遞送，該試劑可實(shí)現(xiàn)靶基因敲低。該試劑并不適合傳遞較小的核酸，如siRNA/miRNA。

實(shí)驗(yàn)數(shù)據(jù)：

Exceptional Transfection Efficiency in Human Induced Pluripotent Stem Cells (iPSCs) via Reverse Transfection with TransIT?-LT1. The TransIT?-LT1 Transfection Reagent was used to reverse transfect 1.3 x 10⁶ iPS cells with a ZsGreen expressing plasmid (Clontech). Reverse transfections were performed in 6-well plates using 12 μl of TransIT?-LT1 Transfection Reagent to deliver 4 μg of DNA (3:1, reagent: DNA). Cells were visualized 48 hours post-transfection and imaged under a 10X objective with an Olympus IX71? Inverted Microscope. Images are (A) phase contrast and (B) green fluorescence. Cells were assayed 48 hours post-transfection on an Accuri? Cytometer. The histogram (C) shows untransfected cells (black line) compared to cells transfected with plasmid using TransIT?-LT1 (green line). 

High Efficiency Transfection of iCell? Cardiomyocytes Using TransIT?-LT1 Transfection Reagent. iCell? Cardiomyocytes were plated at 20,000 cells/well in a 96 well tissue culture plate coated with 0.1% gelatin. After allowing the cells to recover from thaw, cells were transfected with 100 ng/well of pMAXGFP (Lonza) using TransIT?-LT1 Transfection Reagent with a 2:1 reagent-to-DNA ratio according to the manufacturer’s instructions. Fluorescent images were taken 3 days post transfection using a Olympus IX71? inverted microscope. 

he TransIT?-LT1 Reagent Exhibits Higher Expression and Lower Cellular Toxicity Compared to Other Transfection Reagents. HepG2 cells were transfected with a luciferase expression plasmid using the designated reagents at the manufacturer’s recommended reagent-to-DNA ratio indicated beneath each bar. Transfections were performed in 96-well plates using 0.1 μg of plasmid DNA per well. Luciferase expression (bar graph) and lactate dehydrogenase (LDH) levels (line graph) were measured at 24 hours post-transfection. LDH levels are reported as % cytotoxicity compared to cells alone and were measured using a commercially available colorimetric assay; all values at or below zero are represented as zero on graph. Experiments were performed as per industry accepted testing protocols. FuGENE? is a registered trademark of Fugent LLC. Lipofectamine? is a trademark of Life Technologies Corporation.

Comparable Luciferase Expression with the TransIT?-LT1 Reagent and FuGENE? 6 in Multiple Cell Types. The indicated cell lines were transfected in duplicate with 1 μg of a luciferase expression vector per well of a 12-well plate using either 3 μl of the TransIT?-LT1 or FuGENE? 6 Reagents according to industry accepted testing protocols. Cells were harvested 24 hours post-transfection and assayed for luciferase activity. FuGENE? is a registered trademark of Fugent LLC.

注意事項(xiàng)：

1.      為了您的安全和健康，請穿實(shí)驗(yàn)服并戴一次性手套操作。

本產(chǎn)品僅用于生命科學(xué)研究，不得用于醫(yī)學(xué)診斷及其他用途！