MCLA (Chemiluminescent Probe) 超氧化物化學(xué)發(fā)光探針

產(chǎn)品簡(jiǎn)介：

MCLA，又稱(chēng)為6-(4-Methoxyphenyl)-2-methyl-3,7-dihydroimidazo[1,2-a]pyrazin-3(7H)-one hydrochloride，能夠與超氧化物陰離子（O2–）或單線(xiàn)態(tài)氧（1O2)反應(yīng)產(chǎn)生化學(xué)發(fā)光。MCLA與超氧化物可逆反應(yīng)形成一加合物，該加合物的不可逆衰變產(chǎn)生冷光（~465nm），此反應(yīng)表觀(guān)速率常數(shù)是~105M–1s–1。MCLA化學(xué)發(fā)光是靈敏的超氧化物檢測(cè)手段，最顯著的應(yīng)用是白細(xì)胞功能研究。

產(chǎn)品特性：

1）CAS NO.：162558-52-3

2）同義名：2-Methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin

-3(7H)-one hydrochloride, 6-(4-Methoxyphenyl)-2-methyl-3,7-dihydroimidazo[1,2

-a]pyrazin-3(7H)-one hydrochloride, Cypridina Luciferin methoxy-analogue, MCLA

3）分子式：C14H13N3O2· HCl 

4）分子量：291.73

5）純度：≥98.0%

6）Emmax：~465 nm (Chemiluminescence: in 0.1 M Tris pH 8.5; after addition of H2O2)

7）外觀(guān)：白色至淺黃色固體

8）溶解性：溶于水，DMSO，DMF

9）化學(xué)結(jié)構(gòu)圖：

保存條件: 

20oC避光干燥保存，至少1年有效。

產(chǎn)品使用：（僅供參考）

Prepare reaction buffer: 5 μM MCLA + buffer

Buffer examples:  

for Cytochrome C: 50 mM Tris-Cl, pH 7.8, 0.1 mM EDTA, 7 mM sodium succinate .

for mitochondria proteins: 125 mM KCl, 10 mM HEPES, 5 mM MgCl2, 2 mM K2HPO4

Conduct reaction in 100 μl of reaction buffer - Light emission is detected and quantified using a microplate luminometer with opaque (white) 96-well plates. The photomultiplier can be set to default with an integration time of 1000 ms. The MCLA signal is quantified as an integral of 20 s to 60 s of continuous measurement. - A positive control can be prepared with the xanthine/xanthine oxidase system: 1 mM xanthine + 0.1 unit/ml xanthine oxidase causes a ~100-fold increase in chemiluminescent signal as compared with xanthine or xanthine oxidase alone, which did not differ significantly from reaction buffer alone. Addition of SOD (CuZn-SOD from erythrocytes) decreases the MCLA signal by over 98%.

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