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<M1130> Mag-Bind Plant DNA DS 96 Kit 96孔DS磁珠法植物DNA提取試劑盒

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Mag-Bind? Plant DNA DS 96 Kit allows rapid and reliable isolation of high-quality genomic DNA from plants and other tissues that are particularly difficult to lyse or very high in polysaccharide content.

96孔DS磁珠法植物DNA提取試劑盒

Mag-Bind Plant DNA DS 96 Kit


產品編號

產品名稱

包裝規格

目錄價

M1130-00

    96孔DS磁珠法植物DNA提取試劑盒      

1 x 96

1750

M1130-01

96孔DS磁珠法植物DNA提取試劑盒  

4 x 96

6100


產品介紹

Mag-Bind? Plant DNA DS 96 Kit allows rapid and reliable isolation of high-quality genomic DNA from plants and other tissues that are particularly difficult to lyse or very high in polysaccharide content. The lysis and binding buffers are specifically designed to minimize co-purification of polysaccharides and polyphenols. Up to 96 samples of 50 mg wet tissue (or 15 mg dry tissue) can be processed in parallel in less than 1 hour.

The system combines Omega Bio-tek’s E.Z.N.A.? buffer chemistry, which eliminates the need for organic solvents, with the convenience of Mag-Bind? particles to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. This kit is designed for manual or fully automated high-throughput preparation of genomic, chloroplast, and mitochondrial DNA. Purified DNA is suitable for PCR, restriction digestion, Next Generation Sequencing, and hybridization applications. There are no organic extractions, thereby reducing consumables and decreasing hands-on time to allow multiple samples to be processed in parallel.


參數

FEATURES

SPECIFICATIONS

Starting Amount

50 mg wet tissue or 15 mg dry tissue

Starting Material

Plants and other tissues that are particularly difficult to lyse or very high in polysaccharide content.

Elution Volume

100–200 μL

Technology

Magnetic Beads

Processing Mode

Automated, Manual

Throughput

96


組分

ProductNumber

M1130-00

M1130-01

Preparations

1 × 96

4 × 96

CSPL Buffer

80 mL

2 × 150 mL

RBB Buffer

60 mL

240 mL

CSPW1 Buffer

39 mL

143 mL

CSPW2 Buffer

12 mL

44 mL

SPM Buffer

2 × 30 mL

2 × 75 mL

Elution Buffer

60 mL

250 mL

Proteinase K Solution

2.2 mL

8.8 mL

RNase A (25 mg/mL)

1.5 mL

3.2 mL

Mag-Bind? Particles HDQ

2.2 mL

9 mL

User Manual

?

?


流程

  • Homogenize/Lyse

  • Transfer Supernatant, Adjust Binding Conditions

  • Bind DNA with Magnetic Beads

  • Concentrate Magnetic Beads

  • Wash

  • Concentrate Magnetic Beads

  • Dry

  • Elute

  • Transfer Product