用磁珠純化方式高通量地去除自動測序前的游離熒光染料探針 

Mag-Bind SeqDTR

產品介紹

Mag-Bind? SeqDTR is designed to effectively and reliably remove unincorporated terminators from sequencing reactions. The system combines Omega Bio-tek's proprietary chemistries with the reversible nucleic acid-binding properties of magnetic beads to eliminate excess nucleotides, primers, and small, non-targeted amplification products such as primer dimers. Sequencing products are mixed with the Mag-Bind SeqDTR magnetic particles, which selectively bind DNA. Two rapid wash steps eliminate trace contaminants such as nucleotides and primers to reduce background signal and, therefore, achieve higher QV scores.

The high sensitivity of Mag-Bind SeqDTR’s binding ability allows for decreased concentrations of BigDye? chemistry to be used and longer continuous read lengths to be achieved. Mag-Bind SeqDTR can be processed in 96- and 384-well formats and is compatible with many liquid handling instruments, including Hamilton Microlab? STAR? & STARlet?, Beckman Coulter Biomek? FX & NX, and Tecan Evo instruments. Up to 4 plates can be run in a 96-well format in less than 25 minutes.

• No protocol change against major competitor

• Read lengths averaging over 800 bps (Min Phred 20)

• Manual or adjustable to automated liquid handlers

• Significant cost savings compared to sephadex-based clean ups

•  96- or 384-well formats

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