bioGenousTM Mouse Fetal Brain (Differentiation) Organoid Kit is a serum-free culture medium for mouse fetal brain organoids (mFBs). In mouse fetal brain organoids expansion medium, mFBs consist of neural stem cells, characterized by the expression of markers such as Sox2. When the expansion medium is replaced with differentiation medium, mFBs can differentiate into matured neuronal cells, which exhibit specific markers including Gfap, Map2, Cux1, and Dcx. Mouse fetal brain organoids display hallmarks of the mouse fetal brain in terms of architecture, cell type composition, and self-renewal dynamics, therefore hold great promise for studies of mouse brain development and disease.